Daniel J. Ostlie, MD close
Daniel J. Ostlie, MD  

Daniel J. Ostlie, MD Print Friendly Page

Faculty, University of Wisconsin School of Medicine and Public Health

Dr. Ostlie became surgeon-in-chief at American Family Children's Hospital in 2012 after 12 years as a pediatric surgeon at Children's Mercy Hospitals and Clinics in Kansas City. He specializes in minimally invasive pediatric and neonatal surgery, thoracic and oncologic surgery, chest wall deformities, hernia surgery, surgical critical care and trauma and hypospadias repair. Dr. Ostlie completed a two-year Pediatric Surgery Fellowship at the Children's Mercy Hospitals and Clinics in Kansas City and received his certification in Surgical Critical Care after completing the Surgical Critical Care Fellowship. He completed his general surgery residency at the Mayo Clinic Scottsdale, and during that time performed a surgical research fellowship at Cambridge University, Cambridge, England.


Pediatric General Surgery

UW Health Clinics

American Family Children's Hospital
(608) 263-6420 | (800) 323-8942 | Map
American Family Children's Hospital
(608) 263-6420 | (800) 323-8942 | Map

Other Locations

SwedishAmerican Medical Group/Woodside (Rockford)
(779) 696-8499 |  Map

Hospital Affiliation(s)

American Family Children's Hospital (primary)

UW School of Medicine and Public Health

Department of Surgery

Professional Certifications and Education

Board Certification Pediatric Surgery
Surgical Critical Care

Pediatric Surgery and Surgical Critical Care, Children's Mercy Hospital, Kansas City, MO

Residency Mayo Clinic, Scottsdale, AZ
Medical School University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, 1994


Best Doctors® in America 2014

Best Doctors® in America 2013

Medical interpreters are available to help patients communicate with hospital and clinic staff. For more information, please contact interpreter services at (608) 262-9000.


Our doctors provide a wide range of services. The following list represents some, but not all, of the procedures offered by this physician.

Programs & Conditions


A nationally-known expert in minimally-invasive pediatric surgery, Dr. Ostlie's research interests focus on evidence-based pediatric surgery. He has participated as the primary investigator or co-investigator in more than 20 prospective randomized controlled trials in infants and children.

PubMed Articles
Ostlie DJ . 25(th) Anniversary State-of-the-Art Expert Discussion With Carroll McWilliams Harmon, MD, PhD, on Single-Site Surgical Techniques-Past, Present, and Future. J Laparoendosc Adv Surg Tech A. 2015 Feb;25(2):89-93
[PubMed ID: 25683069]
Brinkman AS Rogers AP Acher CW Wynn MM Nichol PF Ostlie DJ Gosain A . Evolution in management of adolescent blunt aortic injuries--a single institution 22-y experience. J Surg Res. 2015 Feb;193(2):523-7
[PubMed ID: 25281286]
Desai AA Ostlie DJ Juang D . Optimal timing of congenital diaphragmatic hernia repair in infants on extracorporeal membrane oxygenation. Semin Pediatr Surg. 2015 Feb;24(1):17-9
[PubMed ID: 25639805]
Smith CD Ostlie DJ . JLAST-Celebrating 25 Years. J Laparoendosc Adv Surg Tech A. 2015 Jan;25(1):1
[PubMed ID: 25607897]
Cunningham JP Knott EM Gasior AC Juang D Snyder CL St Peter SD Ostlie DJ . Is routine chest radiograph necessary after chest tube removal? J Pediatr Surg. 2014 Oct;49(10):1493-5
[PubMed ID: 25280653]
Desai AA Knott EM Alemayehu H Sherman AK St Peter SD Ostlie DJ . Histologic analysis of the hernia sac: current practices based on a survey of IPEG members. J Laparoendosc Adv Surg Tech A. 2014 Sep;24(9):660-3
[PubMed ID: 25115582]
Alemayehu H Snyder CL St Peter SD Ostlie DJ . Incidence and outcomes of unexpected pathology findings after appendectomy. J Pediatr Surg. 2014 Sep;49(9):1390-3
[PubMed ID: 25148744]
Sharp NE Aguayo P Marx DJ Polak EE Rash DE Peter SD Ostlie DJ Juang D . Nursing preference of topical silver sulfadiazine versus collagenase ointment for treatment of partial thickness burns in children: survey follow-up of a prospective randomized trial. J Trauma Nurs. 2014 Sep-Oct;21(5):253-7
[PubMed ID: 25198082]
Hall NJ Eaton S Seims A Leys CM Densmore JC Calkins CM Ostlie DJ St Peter SD Azizkhan RG von Allmen D Langer JC Lapidus-Krol E Bouchard S Piché N Bruch S Drongowski R MacKinlay GA Clark C Pierro A . Risk of incomplete pyloromyotomy and mucosal perforation in open and laparoscopic pyloromyotomy. J Pediatr Surg. 2014 Jul;49(7):1083-6
[PubMed ID: 24952793]
Dodgion CM Gosain A Rogers A St Peter SD Nichol PF Ostlie DJ . National trends in pediatric blunt spleen and liver injury management and potential benefits of an abbreviated bed rest protocol. J Pediatr Surg. 2014 Jun;49(6):1004-8; discussion 1008
[PubMed ID: 24888852]
Ostlie DJ Ponsky TA . Technical options of the laparoscopic pediatric inguinal hernia repair. J Laparoendosc Adv Surg Tech A. 2014 Mar;24(3):194-8
[PubMed ID: 24625350]
Ponsky TA Nalugo M Ostlie DJ . Pediatric laparoscopic inguinal hernia repair: a review of the current evidence. J Laparoendosc Adv Surg Tech A. 2014 Mar;24(3):183-7
[PubMed ID: 24625349]
Ostlie DJ . Minimally invasive pediatric inguinal hernia repair. J Laparoendosc Adv Surg Tech A. 2014 Feb;24(2):59
[PubMed ID: 24512367]
Adibe OO Iqbal CW Sharp SW Juang D Snyder CL Holcomb GW 3rd Ostlie DJ St Peter SD . Protocol versus ad libitum feeds after laparoscopic pyloromyotomy: a prospective randomized trial. J Pediatr Surg. 2014 Jan;49(1):129-32; discussion 132
[PubMed ID: 24439596]
Gasior AC Knott EM Holcomb GW 3rd Ostlie DJ St Peter SD . Patient and parental scar assessment after single incision versus standard 3-port laparoscopic appendectomy: long-term follow-up from a prospective randomized trial. J Pediatr Surg. 2014 Jan;49(1):120-2; discussion 122
[PubMed ID: 24439594]
St Peter SD Aguayo P Juang D Sharp SW Snyder CL Holcomb GW 3rd Ostlie DJ . Follow up of prospective validation of an abbreviated bedrest protocol in the management of blunt spleen and liver injury in children. J Pediatr Surg. 2013 Dec;48(12):2437-41
[PubMed ID: 24314183]
Gasior AC Knott EM Fike FB Moratello VE St Peter SD Ostlie DJ Snyder CL . Ghost publications in the pediatric surgery match. J Surg Res. 2013 Sep;184(1):37-41
[PubMed ID: 23688793]
Sharp NE Pettiford-Cunningham J Shah SR Thomas P Juang D St Peter SD Ostlie DJ . The prevalence of Hirschsprung disease in premature infants after suction rectal biopsy. J Surg Res. 2013 Sep;184(1):374-7
[PubMed ID: 23647803]
Ostlie DJ Sharp NE Thomas P Sharp SW Holcomb GW 3rd St Peter SD . Patient scar assessment after single-incision versus four-port laparoscopic cholecystectomy: long-term follow-up from a prospective randomized trial. J Laparoendosc Adv Surg Tech A. 2013 Jun;23(6):553-5
[PubMed ID: 23731081]
Knott EM Gasior AC Ostlie DJ Holcomb GW 3rd St Peter SD . Decreased resource utilization since initiation of institutional clinical pathway for care of children with perforated appendicitis. J Pediatr Surg. 2013 Jun;48(6):1395-8
[PubMed ID: 23845636]
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